Developmental Dynamics SUGIMOTO LAB.
2024
Tamagawa ,K., Dayi, M., Sun, S., Hata, R., Kikuchi, T., Haruta, N., Sugimoto, A., Makino, T.,(2024)
Sci Adv.2024 Sep 13;10(37):eadn9913,(2024), doi: 10.1126/sciadv.adn9913,PubMed
2023
Nami, H., Eisuke S., Yu, H., Masahiro, T., Chihiro, U., Mika ,T., Yukihiko, K., Asako, S. (2023)
A germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans.
J. Cell. Sci. 136(13), jcs260922. PubMed
Kazuma, K., Taruho I., Ryusei, T., Mehmet, D., Takashi, M., Shinichiro ,M., Taisei, K., Asako, S., and Masakado, K. (2023)
Differentially Expressed Genes Associated with Body Size Changes and Transposable Element Insertions between Caenorhabditis elegans and Its Sister Species, Caenorhabditis inopinata. Genome Biol. Evol. 15(4), evad063. PubMed
Yugeta, A., Arai, H., Takahashi, D., Haruta, N., Sugimoto, A., and *Arimoto, H., (2023)
C. elegans ATG-5 mutants associated with ataxia, MicroPub. Bio, (2023) 10.17912/micropub.biology.000792., PubMed
2022
Nakajo, M., Kano, H., Tsuyama, K., Haruta, N. and Sugimoto, A. (2022) Centrosome maturation requires phosphorylation-mediated sequential domain interactions of SPD-5.J. Cell. Sci.,135(8), jcs259025. PubMed
Oomura, S., Tsuyama, K., Haruta, N., and Sugimoto A. (2022) Transgenesis of the gonochoristic nematodeCaenorhabditis inopinataby microparticle bombardment with hygromycin B selection.MicroPub. Biol. PubMed
Negishi, T., Kitagawa, S., Horii. N., Tanaka, Y., Haruta, N., Sugimoto, A., Sawa, H., Hayashi, K., Harata, M. and Kanemaki, MT_cc781905- 5cde-3194-bb3b-136bad5cf58d_(2022) The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development inCaenorhabditis elegans.Genetics PubMed
2021
Nishida, K., Tsuchiya, K., Obinata, H., Onodera, S., Honda, Y., Lai, Y.-C., Haruta, N. and Sugimoto, A.(2021) Expression Patterns and Levels of All Tublin Isotypes Analyzed in GFP Knock-InC. elegansStrains.Cell Struct. Funct.;46(1):51-64. PubMed
2019
Yanagi Shota, Sugai Tomoya, Noguchi Takuma, Kawakami Masao, Sasaki Makoto, Niwa Shinsuke, Sugimoto Asako, Fuwa Haruhiko(2019) Fluorescence-labeled neopeltolide derivatives for subcellular localization imaging.
Organic & Biomolecular Chemistry PubMed
2018
Namai S, Sugimoto A.(2018) Transgenesis by microparticle bombardment for live imaging of fluorescent proteins inPristionchus pacificusgermlines and early embryos.Dev. Genes Evol.Jan., Volume 228, Issue 1, pp 75–82 PubMed
Obinata H, Sugimoto A, Niwa S.(2018) Streptothricin acetyl transferase 2 (Sat2): A dominant selection marker forCaenorhabditis elegansgenome editing.PLoS ONE,13(5):e0197128 PubMed
Kanzaki N, Tsai IJ, Tanaka R, Hunt VL, Liu D, Tsuyama K, Maeda Y, Namai S, Kumagai R, Tracey A, Holroyd N, Doyle SR, Woodruff GC, Murase K, Kitazume H, Chai C, Akagi A , Panda O, Ke HM, Schroeder FC, Wang J, Berriman M, Sternberg PW, Sugimoto A, Kikuchi T.(2018) Biology and genome of a newly discovered sibling species ofCaenorhabditis elegans.
Nat.Commun., 10;9(1):3216 PubMed, Commentary, news
2017
Honda Y., Tsuchiya K., Sumiyoshi E., Haruta N., Sugimoto A.Tubulin isotype substitution revealed that isotype composition modulates microtubule dynamics inC. elegans embryos. J Cell Sci. PubMed
水島昇, 杉本亜砂子, 吉森保, 岩崎秀雄. 『いま学会・科学コミュニティのあり方を議論しよう.』
実験医学 Vol.35 No.13, 2216-2225.
2015
Sumiyoshi, E., Fukata, Y., Namai, S., Sugimoto, A. Caenorhabditis elegansAurora A kinase is required for the formation of spindle microtubules in female meiosis.Mol Biol Cell.26(23):4187-96. PubMed
2014
Kubota, Y., Tsuyama, K., Takabayashi, Y., Haruta, N., Maruyama, R., Iida, N., Sugimoto, A.The PAF1 complex is involved in embryonic epidermal morphogenesis inCaenorhabditis elegans.Dev. Biol. 391(1):43-53.PubMed
2013
Kress, E., Schwager, F., Holtackers, R., Seiler, J., Prodon, F., Zanin, E., Eiteneuer, A., Toya, M., Sugimoto, A., Meyer, H., Meraldi, P., Gotta, M. (2013). The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A.J Cell Biol. 201, 559-575. PubMed
Doi, M., Minematsu, H., Kubota, Y., Nishiwaki, K., and Miyamoto, M.(2013). The novel Rac effector RIN-1 regulates neuronal cell migration and axon pathfinding inC. elegans.Development140, 3435-3444. PubMed
杉本亜砂子 (2013). 『細胞分裂131年目の真実ー分子から動態へ：基礎の基礎.』 細胞工学 32, 258-262.
住吉英輔, 杉本亜砂子 (2013). 『体細胞分裂と減数分裂における紡錘体形成.』 細胞工学 32, 269-274.
2012
Sumiyoshi, E., and Sugimoto, A.(2012). Cell polarity: centrosomes release signals for polarization.Curr. Biol. 22, R281-283.
Kubota, Y., Nagata, K., Sugimoto, A., and Nishiwaki, K.(2012).Caenorhabditis elegansgonad depends on interactions among fibulin-1, type IV collagen and the ADAMTS extracellular protease.Genetics190, 1379-1388. PubMed
Hachet, V., Busso, C., Toya, M., Sugimoto, A., Askjaer, P., and Gonczy, P.(2012). The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process inCaenorhabditis elegansembryos.
Mol. Biol. Cell23, 3111-3121. PubMed
2011
Toya, M., Terasawa, M., Nagata, K., Iida, Y., and Sugimoto, A.(2011). A kinase-independent role for Aurora A in the assembly of mitotic spindle microtubules inCaenorhabditis elegans embryos. Nat. Cell.13, 710-716. PubMed
Sumiyoshi, E., Takahashi, S., Obata, H., Sugimoto, A., and Kohara, Y.(2011). The beta-catenin HMP-2 functions downstream of Src in parallel with the Wnt pathway in early embryogenesis ofC. elegans.Dev. Biol.355, 302-312. PubMed
Sugimoto, A.(2011). Toward the second stage of recovery from the 3.11 Tohoku Earthquake.
Genes Cells16, 745-747. PubMed
Hanazawa, M., Yonetani, M., and Sugimoto, A.(2011). PGL proteins self associate and bind RNPs to mediate germ granule assembly inC. elegans.J Cell Biol.192, 929-937. PubMed
2010
Toya, M., Iida, Y., and Sugimoto, A.(2010). Imaging of mitotic spindle dynamics inCaenorhabditis elegansembryos.
Methods Cell Biol. 97, 359-372.PubMed
Terasawa, M., Toya, M., Motegi, F., Mana, M., Nakamura, K., and Sugimoto, A.(2010).Caenorhabditis elegansortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin.Genes Cells15, 1145-1157. PubMed
Ogawa, H., Shionyu, M., Sugiura, N., Hatano, S., Nagai, N., Kubota, Y., Nishiwaki, K., Sato, T., Gotoh, M., Narimatsu, H., Shimizu, K., Kimata, K., Watanabe, H.(2010). Chondroitin sulfate synthase-2/chondroitin polymerizing factor has two variants with distinct functions.J Biol. Chem.285, 34155-34167. PubMed
Kikuchi, T., Kubota, Y., Ihara, S., and Nishiwaki, K.(2010).C. elegans] Seikagaku 82, 957-962. PubMed
- 2009
花澤桃世, 米谷匡史, 杉本亜砂子 (2009). 『線虫における生殖顆粒の形成と配分』
(Germ granule formation and distribution in C.elegans). 蛋白質 核酸 酵素 54, 2147-2152.
Sugimoto, A. (2009). Clearance of germ granules in the soma. F1000 Biology Reports 2009 1:49.
Han, X., Gomes, J.E., Birmingham, C.L., Pintard, L., Sugimoto, A., and Mains, P.E. (2009). The role of protein phosphatase 4 in regulating microtubule severing in the Caenorhabditis elegans embryo. Genetics 181, 933-943.
Takeda, K., Watanabe, C., Qadota, H., Hanazawa, M., and Sugimoto, A. (2008). Efficient production of monoclonal antibodies recognizing specific structures in Caenorhabditis elegans embryos using an antigen subtraction method.
Genes Cells 13, 653-665.
Konishi, T., Uodome, N., and Sugimoto, A. (2008). The Caenorhabditis elegans DDX-23, a homolog of yeast splicing factor PRP28, is required for the sperm-oocyte switch and differentiation of various cell types. Dev. Dyn. 237, 2367-2377.
Gassmann, R., Essex, A., Hu, J.S., Maddox, P.S., Motegi, F., Sugimoto, A., O'Rourke, S.M., Bowerman, B., McLeod, I., Yates, J.R., 3rd, et al. (2008). A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex. Genes & development 22, 2385-2399.
茂木文夫, 杉本亜砂子 (2007). 対称から非対称へ：線虫受精卵における細胞極性確立メカニズム. 細胞工学 26, 294-295.
杉本亜砂子 (2007). 線虫の比較ゲノム. In 比較ゲノム学から読み解く生命システム, 藤山秋佐夫, ed. (東京, 秀潤社), pp. 105-110.
杉本亜砂子 (2007). ゲノムに書かれた発生プログラムを読み解くーー線虫をモデル系として. In ゲノムは何をどのように決めているのか？ 生命システムの理解へ向けて, 文部科学省科学研究費特定領域研究「ゲノム4領域」, ed. (東京, クバプロ), pp. 101-112.
杉本亜砂子 (2007). 体系的表現型解析で探る線虫の発生機構. 実験医学 25, 151-157.
小西隆文, and 杉本亜砂子 (2007). in vivoイメージングのためのプローブ. In 実験がうまくいく蛍光・発光試薬の選び方と使い方, 三輪佳宏, ed. (東京, 羊土社), pp. 114-118.
Maruyama, R., Velarde, N.V., Klancer, R., Gordon, S., Kadandale, P., Parry, J.M., Hang, J.S., Rubin, J., Stewart-Michaelis, A., Schweinsberg, P., et al. (2007). EGG-3 regulates cell-surface and cortex rearrangements during egg activation in Caenorhabditis elegans. Curr. Biol. 17, 1555-1560.
茂木文夫, 杉本亜砂子 (2006). 細胞質分裂の開始における微小管のはたらき. 蛋白質核酸酵素 51, 1590-1595.
茂木文夫, 杉本亜砂子 (2006). 線虫初期胚の非対称分裂から学ぶ"極性化". 蛋白質核酸酵素 51, 776-781.
杉本亜砂子 (2006). ノーベル賞受賞者の業績と人柄：生理学・医学賞 RNA干渉：二本鎖RNAによる新しい遺伝子発現抑制機構の発見. 科学 77, 13-15.
杉本亜砂子 (2006). 2006年ノーベル生理学・医学賞は"RNA干渉"の発見に. Bionics 4, 10-11.
杉本亜砂子 (2006). 折り返し地点に立って思うこと 接尾語としての-Biology. 蛋白質核酸酵素 51, 1696?1698.
Motegi, F., Velarde, N.V., Piano, F., and Sugimoto, A. (2006). Two phases of astral microtubule activity during cytokinesis in
C. elegans embryos. Dev. Cell. 10, 509-520.
Motegi, F., and Sugimoto, A. (2006). Sequential functioning of the ECT-2 RhoGEF, RHO-1 and CDC-42 establishes cell polarity in Caenorhabditis elegans embryos. Nat. Cell Biol. 8, 978-985.
飯田直子, 杉本亜砂子 (2005). 線虫（C. elegans）におけるRNAiの応用. In RNA工学の最前線, 中村義一, and 大内将司, eds. (東京, シーエムシー出版), pp. 41-51.
杉本亜砂子, 小原雄治 (2005). 線虫ゲノムの体系的発現・機能解析. 蛋白質核酸酵素 50, 2140-2145.
Yanagi, K., Mizuno, T., Tsuyama, T., Tada, S., Iida, Y., Sugimoto, A., Eki, T., Enomoto, T., and Hanaoka, F. (2005). Caenorhabditis elegans geminin homologue participates in cell cycle regulation and germ line development. J Biol. Chem. 280, 19689-19694.
Sugimoto, A. (2005). WormMethod, Reverse genetics: RNAi by soaking. In WormBook, T.C.e.R. Community, ed.
Sugimoto, A. (2005). High-throughput RNAi-by-soaking in Caenorhabditis elegans. In RNA interference technology: From basic science to drug development, K. Appasani, ed. (Cambridge, Cambridge University Press), pp. 419-432.
Maruyama, R., Hayakawa, C., Iida, N., Mochizuki, Y., Toyoda, T., and Sugimoto, A. (2005). Profiling and clustering of developmental phenotypes in Caenorhabditis elegans. submitted.
Maruyama, R., Endo, S., Sugimoto, A., and Yamamoto, M. (2005). Caenorhabditis elegans DAZ-1 is expressed in proliferating germ cells and directs proper nuclear organization and cytoplasmic core formation during oogenesis. Dev. Biol. 277, 142-154.
Furuya, M., Qadota, H., Chisholm, A.D., and Sugimoto, A. (2005). The C. elegans eyes absent ortholog EYA-1 is required for tissue differentiation and plays partially redundant roles with PAX-6. Dev Biol 286, 452-463.
飯田直子, 魚留信子, 杉本亜砂子, 門田裕志 (2004). 生物種別RNAi実験プロトコール：線虫. In 改訂 RNAi実験プロトコール, 多比良和誠, 宮岸真, 川崎広明, 明石英雄, eds. (東京, 羊土社), pp. 45-55.
杉本亜砂子 (2004). 動物のゲノム解析：線虫. In ゲノミクス・プロテオミクスの新展開, 今中忠行, ed. (東京, エヌ・ティー・エス), pp. 338-346.
Sugimoto, A. (2004). High-throughput RNAi in Caenorhabditis elegans: genome-wide screens and functional genomics. Differentiation 72, 81-91.
Inoue, T., Sugimoto, A., Suzuki, Y., Yamamoto, M., Tsujimoto, M., Inoue, K., Aoki, J., and Arai, H. (2004). Type II platelet-activating factor-acetylhydrolase is essential for epithelial morphogenesis in Caenorhabditis elegans. Proc Natl Acad Sci U S A 101, 13233-13238.
杉本亜砂子, 門田裕志 (2003). RNA干渉法. In 線虫ラボマニュアル, 三谷昌平, ed. (東京, シュプリンガー・フェアラーク東京), pp. 115-133.
杉本亜砂子 (2003). 線虫C. elegansのゲノム機能解析. Paper presented at: 第27回阿蘇シンポジウム 2003「医学をゲノムする」 (熊本, 南山堂).
杉本亜砂子 (2003). プログラム細胞死. In 線虫 究極のモデル生物, 飯野雄一, and 石井直明, eds. (東京, シュプリンガー・フェアラーク東京), pp. 45-55.
杉本亜砂子 (2003). 2002年ノーベル医学生理学賞 線虫C. elegans研究とプログラム細胞死. 現代化学 382, 20-23.
作本直子, 魚留信子, 杉本亜砂子, 門田裕志 (2003). 生物種別RNAi実験プロトコール：線虫. In RNAi実験プロトコール, 多比良和誠, 宮岸真, 川崎広明, and 明石英雄, eds. (東京, 羊土社), pp. 37-47.
Sawa, M., Suetsugu, S., Sugimoto, A., Miki, H., Yamamoto, M., and Takenawa, T. (2003). Essential role of the C. elegans Arp2/3 complex in cell migration during ventral enclosure. J Cell Sci. 116, 1505-1518.
Mito, Y., Sugimoto, A., and Yamamoto, M. (2003). Distinct Developmental Function of Two Caenorhabditis elegans Homologs of the Cohesin Subunit Scc1/Rad21. Mol. Biol. Cell 14, 2399-2409.
杉本亜砂子 (2002). 線虫C. elegans研究の創始者たち3人にノーベル賞. 実験医学 20, 2643-2644.
杉本亜砂子 (2002). RNAi---その分子機構と応用. 化学と生物 40, 713-718.
杉本亜砂子 (2002). ゲノム工学：RNAi. ゲノム医学 2, 181-186.
前田郁麻, 杉本亜砂子 (2002). RNAiによる線虫遺伝子機能の体系的解析. 分子細胞治療 1, 121-124.
Sumiyoshi, E., Sugimoto, A., and Yamamoto, M. (2002). Protein phosphatase 4 is required for centrosome maturation in mitosis and sperm meiosis in C. elegans. J Cell Sci. 115, 1403-1410.
Kodama, Y., Rothman, J.H., Sugimoto, A., and Yamamoto, M. (2002). The stem-loop binding protein CDL-1 is required for chromosome condensation, progression of cell death and morphogenesis in Caenorhabditis elegans. Development 129, 187-196.
杉本亜砂子 (2001). 体系的RNA干渉法による線虫C. elegansの発生に必須な遺伝子群の探索. 医学のあゆみ 199, 9945-9949.
杉本亜砂子 (2001). 線虫ゲノムの機能解析. In ゲノムから個体へ 生命システムの理解に向けて, 榊佳之, and 小原雄治, eds. (東京, 中山書店), pp. 16-29.
杉本亜砂子 (2001). 他の生物におけるゲノム解析の現状と未来：線虫. In 植物のゲノム研究プロトコール, 佐々木卓治, 田畑哲之, and 島本功, eds. (東京, 秀潤社), pp. 237-239.
前田郁麻, and 杉本亜砂子 (2001). 線虫ゲノムの体系的機能解析. 蛋白質核酸酵素 46, 2432-2435.
Yoshida, M., Sugimoto, A., Ohshima, Y., and Takeshima, H. (2001). Important role of junctophilin in nematode motor function. Biochem Biophys Res Commun 289, 234-239.
Sugimoto, A., Kusano, A., Hozak, R.R., Derry, W.B., Zhu, J., and Rothman, J.H. (2001). Many genomic regions are required for normal embryonic programmed cell death in Caenorhabditis elegans. Genetics 158, 237-252.
Maeda, I., Kohara, Y., Yamamoto, M., and Sugimoto, A. (2001). Large-scale analysis of gene function in Caenorhabditis elegans by high- throughput RNAi. Curr. Biol. 11, 171-176.
辛島健, 杉本亜砂子 (2000). 線虫C. elegansにおける生殖細胞形成過程の逆遺伝学的解析. 細胞工学 19, 913-921.
杉本亜砂子 (2000). 線虫における生殖細胞の形成. In 生殖細胞 その誕生と振る舞い, 第14回「大学と科学」公開シンポジウム組織委員会, ed. (東京, クバプロ), pp. 33-43.
杉本亜砂子 (2000). 線虫をモデル系とした細胞死研究の進展. Molecular Medicine 37, 446-452.
Karashima, T., Sugimoto, A., and Yamamoto, M. (2000). Caenorhabditis elegans homologue of the human azoospermia factor DAZ is required for oogenesis but not for spermatogenesis. Development 127, 1069-1079.
杉本亜砂子 (1999). C. elegansについて知っている2、3の事柄. In 実験室の小さな生きものたち, 羊土社「実験医学」編集部, ed. (東京, 羊土社), pp. 24-28.
杉本亜砂子 (1999). 線虫. In 新アポトーシス実験法, 辻本賀英, 刀祢重信, and 山田武, eds. (東京, 羊土社), pp. 158-163.
Ohmachi, M., Sugimoto, A., Iino, Y., and Yamamoto, M. (1999). kel-1, a novel Kelch-related gene in Caenorhabditis elegans, is expressed in pharyngeal gland cells and is required for the feeding process. Genes Cells 4, 325-337.
杉本亜砂子 (1998). 線虫C. elegansのプログラム細胞死. 医学のあゆみ 187, 447-451.
杉本亜砂子 (1998). ネマトーダモデルとアポトーシス関連遺伝子. In アポトーシスと疾患「基礎編」, 井川洋二, ed. (大阪, 医薬ジャーナル社), pp. 32-43.
杉本亜砂子 (1998). C. elegansについて知っている2、3の事柄. 実験医学 16, 549.
Sugimoto, A., and Miura, M. (1998). Death signalling in C. elegans and activation mechanisms of caspases. In Apoptosis genes, C.S. Potten, C. Booth, and J.W. Wilson, eds. (Norwell, MA, Kluwer Academic Publishers), pp. 167-203.
杉本亜砂子 (1997). 線虫におけるプログラム細胞死の分子機構. In アポトーシス研究の新展開, 米原伸, ed. (東京化学同人), pp. 3-12.
杉本亜砂子 (1997). モデル系としてのC. elegans ?アポトーシス制御機構の種を越えた普遍性. 実験医学 15, 65-69.
Zhu, J., Hill, R.J., Heid, P.J., Fukuyama, M., Sugimoto, A., Priess, J.R., and Rothman, J.H. (1997). end-1 encodes an apparent GATA factor that specifies the endoderm precursor in Caenorhabditis elegans embryos. Genes & development 11, 2883-2896.
Kitayama, C., Sugimoto, A., and Yamamoto, M. (1997). Type II myosin heavy chain encoded by the myo2 gene composes the contractile ring during cytokinesis in Schizosaccharomyces pombe. J Cell Biol. 137, 1309-1319.
杉本亜砂子 (1995). アポトーシスの諸過程を解析する手段 (4) 遺伝学的手法 a) C. elegans. In 最新アポトーシス実験法, 辻本賀英, 刀祢重信, and 山田武, eds. (羊土社), pp. 217-226.
Sugimoto, A., Hozak, R.R., Nakashima, T., Nishimoto, T., and Rothman, J.H. (1995). dad-1, an endogenous programmed cell death suppressor in Caenorhabditis elegans and vertebrates. EMBO J 14, 4434-4441.
Kunitomo, H., Sugimoto, A., Wilkinson, C.R., and Yamamoto, M. (1995). Schizosaccharomyces pombe pac2+ controls the onset of sexual development via a pathway independent of the cAMP cascade. Curr Genet 28, 32-38.
Kanoh, J., Sugimoto, A., and Yamamoto, M. (1995). Schizosaccharomyces pombe zfs1+ encoding a zinc-finger protein functions in the mating pheromone recognition pathway. Mol. Biol. Cell 6, 1185-1195.
Sugimoto, A., Friesen, P.D., and Rothman, J.H. (1994). Baculovirus p35 prevents developmentally programmed cell death and rescues a ced-9 mutant in the nematode Caenorhabditis elegans. EMBO J 13, 2023-2028.
杉本亜砂子, 山本正幸 (1991). 減数分裂. In Annual Review 細胞生物学1991, 矢原一郎, 御小柴克彦, and 月田承一郎, eds. (中外医学社), pp. 181-191.
Sugimoto, A., Iino, Y., Maeda, T., Watanabe, Y., and Yamamoto, M. (1991). Schizosaccharomyces pombe ste11+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual development. Genes & development 5, 1990-1999.
Iino, Y., Sugimoto, A., and Yamamoto, M. (1991). S. pombe pac1+, whose overexpression inhibits sexual development, encodes a ribonuclease III-like RNase. EMBO J 10, 221-226.
Meshi, T., Watanabe, Y., Saito, T., Sugimoto, A., Maeda, T., and Okada, Y. (1987). Function of the 30kd protein of tobacco mosaic virus: involvement in cell-to cell movement and dispensability for replication. EMBO J 6, 2557-2563.